Journal: bioRxiv

Article Title: Micro and nanoplastic inhalation during pregnancy elicits uterine endothelial dysfunction in Sprague Dawley rats by impeding nitric oxide signaling

doi: 10.1101/2025.09.12.675811

Figure Lengend Snippet: Vascular reactivity of the uterine artery was assessed via wire myography. Concentration-response curves were generated after treatment of uterine artery segments with increasing concentrations of the endothelial-dependent vasodilator methacholine (A), the endothelial-independent vasodilator sodium nitroprusside (B), and the vasoconstrictor phenylephrine (C). Significance was assessed by comparing overall reactivity via a four-parameter nonlinear regression analysis. Data are presented as mean ± SEM, n =7-10.

Article Snippet: Endothelium-dependent relaxation, endothelium-independent relaxation, and smooth muscle contraction were separately evaluated via cumulative additions of 60 μL of Methacholine chloride (MCh, MP Biomedicals, CAT 190231), Sodium Nitroprusside Dihydrate (SNP, Sigma-Aldrich, CAT 567538), and Phenylephrine (PE, Thermofisher Scientific, CAT 207240100), respectively for a concentration-response curve spanning from 10 − 9 to 10 − 4 M for each pharmacological application.

Techniques: Concentration Assay, Generated

Journal: bioRxiv

Article Title: Micro and nanoplastic inhalation during pregnancy elicits uterine endothelial dysfunction in Sprague Dawley rats by impeding nitric oxide signaling

doi: 10.1101/2025.09.12.675811

Figure Lengend Snippet: Vascular reactivity of the radial artery was assessed via pressure myography. Concentration-response curves were generated after treatment of radial artery segments with increasing concentrations of the endothelial-dependent vasodilator methacholine (A), the endothelial-independent vasodilator sodium nitroprusside (B), and the vasoconstrictor phenylephrine (C). Significance was assessed by comparing overall reactivity via a four-parameter nonlinear regression analysis. Data are presented as mean ± SEM, n =8-10. Significant differences (p < 0.05) from the control group are denoted by * and significant differences from the polyamide-exposed group are denoted by †.

Article Snippet: Endothelium-dependent relaxation, endothelium-independent relaxation, and smooth muscle contraction were separately evaluated via cumulative additions of 60 μL of Methacholine chloride (MCh, MP Biomedicals, CAT 190231), Sodium Nitroprusside Dihydrate (SNP, Sigma-Aldrich, CAT 567538), and Phenylephrine (PE, Thermofisher Scientific, CAT 207240100), respectively for a concentration-response curve spanning from 10 − 9 to 10 − 4 M for each pharmacological application.

Techniques: Concentration Assay, Generated, Control

Journal: bioRxiv

Article Title: Micro and nanoplastic inhalation during pregnancy elicits uterine endothelial dysfunction in Sprague Dawley rats by impeding nitric oxide signaling

doi: 10.1101/2025.09.12.675811

Figure Lengend Snippet: Myoglobin oxidation by live uterine vasculature was measured as a surrogate for NO-related species production. Oxymyoglobin oxidation was measured at 3 minutes (A) and 15 minutes (B) after stimulation of vascular tissue with methacholine. Significance was assessed through two-tailed Student’s T-Test. Data are presented as mean ± SEM, n =9. Significant differences (p < 0.05) between the control and polyamide-exposed groups are denoted by *.

Article Snippet: Endothelium-dependent relaxation, endothelium-independent relaxation, and smooth muscle contraction were separately evaluated via cumulative additions of 60 μL of Methacholine chloride (MCh, MP Biomedicals, CAT 190231), Sodium Nitroprusside Dihydrate (SNP, Sigma-Aldrich, CAT 567538), and Phenylephrine (PE, Thermofisher Scientific, CAT 207240100), respectively for a concentration-response curve spanning from 10 − 9 to 10 − 4 M for each pharmacological application.

Techniques: Two Tailed Test, Control

Journal: bioRxiv

Article Title: Micro and nanoplastic inhalation during pregnancy elicits uterine endothelial dysfunction in Sprague Dawley rats by impeding nitric oxide signaling

doi: 10.1101/2025.09.12.675811

Figure Lengend Snippet: The effect of L-arginine supplementation on radial endothelium-dependent vasodilation and arginase protein expression in whole uterine vasculature was assessed. After incubation of radial artery segments with L-arginine, concentration-response curves were generated via treatment of segments with increasing concentrations of the endothelial-dependent vasodilator methacholine (A). Relative protein expression of arginase 1 in uterine vasculature was determined by Western Blot (B). Significance was assessed by comparing overall reactivity via a four-parameter nonlinear regression or two-tailed Student’s t-test analysis for vascular reactivity and Western Blot, respectively. Data are presented as mean ± SEM, n =8-9. Significant differences (p < 0.05) from the control group is denoted by *.

Article Snippet: Endothelium-dependent relaxation, endothelium-independent relaxation, and smooth muscle contraction were separately evaluated via cumulative additions of 60 μL of Methacholine chloride (MCh, MP Biomedicals, CAT 190231), Sodium Nitroprusside Dihydrate (SNP, Sigma-Aldrich, CAT 567538), and Phenylephrine (PE, Thermofisher Scientific, CAT 207240100), respectively for a concentration-response curve spanning from 10 − 9 to 10 − 4 M for each pharmacological application.

Techniques: Expressing, Incubation, Concentration Assay, Generated, Western Blot, Two Tailed Test, Control

Journal: bioRxiv

Article Title: Micro and nanoplastic inhalation during pregnancy elicits uterine endothelial dysfunction in Sprague Dawley rats by impeding nitric oxide signaling

doi: 10.1101/2025.09.12.675811

Figure Lengend Snippet: The effect of BH 4 supplementation on radial endothelium-dependent vasodilation and GTPCH and DHFR protein expression in whole uterine vasculature was assessed. After incubation of radial artery segments with BH 4 , concentration-response curves were generated via treatment of segments with increasing concentrations of the endothelial-dependent vasodilator methacholine (A). Relative protein expression of GTPCH (B) and DHFR (C) in uterine vasculature was determined by Western Blot. Significance was assessed by comparing overall reactivity via a four-parameter nonlinear regression or two-tailed Student’s t-test analysis for vascular reactivity and Western Blot, respectively. Data are presented as mean ± SEM, n =8-9. Significant differences (p < 0.05) from the control group are denoted by *. Significant differences (p < 0.05) from the polyamide-exposed group are denoted by †.

Article Snippet: Endothelium-dependent relaxation, endothelium-independent relaxation, and smooth muscle contraction were separately evaluated via cumulative additions of 60 μL of Methacholine chloride (MCh, MP Biomedicals, CAT 190231), Sodium Nitroprusside Dihydrate (SNP, Sigma-Aldrich, CAT 567538), and Phenylephrine (PE, Thermofisher Scientific, CAT 207240100), respectively for a concentration-response curve spanning from 10 − 9 to 10 − 4 M for each pharmacological application.

Techniques: Expressing, Incubation, Concentration Assay, Generated, Western Blot, Two Tailed Test, Control

Journal: bioRxiv

Article Title: Micro and nanoplastic inhalation during pregnancy elicits uterine endothelial dysfunction in Sprague Dawley rats by impeding nitric oxide signaling

doi: 10.1101/2025.09.12.675811

Figure Lengend Snippet: The effect of DTT on radial endothelium-dependent vasodilation function and 3-nitrotyrosine, thioredoxin, and GCLC protein expression in whole uterine vasculature was assessed. After incubation of radial artery segments with DTT, concentration-response curves were generated via treatment of segments with increasing concentrations of the endothelial-dependent vasodilator methacholine (A). Relative protein expression of 3-nitrotyrosine residues (B) thioredoxin (C) and GCLC (D) in uterine vasculature was determined by Western Blot. Significance was assessed by comparing overall reactivity via a four-parameter nonlinear regression or two-tailed Student’s t-test analysis for vascular reactivity and Western Blot, respectively. Data are presented as mean ± SEM, n =8-9. Significant differences (p < 0.05) from the control group are denoted by *. Significant differences (p < 0.05) from the polyamide-exposed group are denoted by †.

Article Snippet: Endothelium-dependent relaxation, endothelium-independent relaxation, and smooth muscle contraction were separately evaluated via cumulative additions of 60 μL of Methacholine chloride (MCh, MP Biomedicals, CAT 190231), Sodium Nitroprusside Dihydrate (SNP, Sigma-Aldrich, CAT 567538), and Phenylephrine (PE, Thermofisher Scientific, CAT 207240100), respectively for a concentration-response curve spanning from 10 − 9 to 10 − 4 M for each pharmacological application.

Techniques: Expressing, Incubation, Concentration Assay, Generated, Western Blot, Two Tailed Test, Control

Journal: Science Advances

Article Title: Functional vascularized lung grafts for lung bioengineering

doi: 10.1126/sciadv.1700521

Figure Lengend Snippet: Preservation of bronchial structure of native and de-epithelialized lungs ( A to F ). Pentachrome stain (A and B). SMA immunofluorescence staining (C and D). Airways are indicated by arrows, and vessels are indicated by stars. Scanning electron microscopy of airway casts (E and F). ( G and H ) Airway responsiveness during intravascular administration of methacholine measured by pressure-volume loops. Lung compliance in native (G) (0.190 ± 0.046 ml/cmH 2 O; after methacholine, 0.048 ± 0.007 ml/cmH 2 O; reduction of ~73.48 ± 8.704%) and de-epithelialized (H) (0.065 ± 0.036ml/cmH 2 O; after methacholine, 0.031 ± 0.004 ml/cmH 2 O; reduction of 45.28 ± 18.71%) lungs. Values are expressed as means ± SD.

Article Snippet: The effect of methacholine chloride (Methapharm) on bronchial muscular tone was tested in de-epithelialized ( n = 3) and control ( n = 3) rats.

Techniques: Preserving, Staining, Immunofluorescence, Electron Microscopy